女性ホルモンのラツト子宮粘膜に及ぼす組織学的影響
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概要
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Fifty-two adult female rats of Wistar strain were used for this investigation. Animals were devided into such experimental groups as control, castrated, estrogen injection, progesterone administration, constant oestrus and constant dioestrus. Cytological observations were made on the mucous membrane of uterus, with special remarks of the endometrial epithelium and uterine gland. Estrogen administration (estradiol benz. 0.05 mg., estrone 8 gamma per day for 10, 40 days) increased the organ bodyweight ratio of uterus both in intact and castrated rats, 18 times in the former and 2.6 times in the latter. Progesterone injection (2 mg. per day for 10 days) also increased organ body-weight ratio 2.6 times.<BR>Ten days after the castration, the cavum uteri was reduced in size and the mucous membrane was also atrophic, in which the epithelial cells and uterine gland cells were provided with low columar or cuboidal feature coincident in their internal structure with dioestrus. Within the advancing postoperative days (45,365 days), atrophic changes were more profound both in the epithelial cells making the plane luminal surface and in lining cells of uterine gland among which the retention cyste was frequently recognized. In constant dioestrus, the low epithelial cells also manifested the corresponding signs to those caused by the presistent castration status.<BR>Estrogen administration to intact or castrated rats induced the pronounced hypertrophy, hyperplasia and elongation of the epithelial cells in which were present the abundant mitochondria and huge vacuoles at the basal part followed by the deformation of nuclear membrane ; a certain changes caused by the castration were completely recovered to be normal or rather of hyperfunctional condition. By the administration the epithelial cells were, in case of remaining of bilateral ovaries, the thinest and tallest, therefore they exceeded the presentations in prooestrus and oestrus, in spite of the obscure discernment for the apocrine secretion ; in the castrated rats the extension in length of the epithelial cells was also preferable to an elongation seen in prooestrus, and might be conditioned on amount and period of administration of the chemicals ; they consisted commonly of pale cells characterized by the occurrence of stainable granules (PAS-positive) chiefly at the supranuclear portions, sometimes associated with a few number of dark hyperchromatic cells ; between cells there were occasionally observed the deformed lymphocytes ; the picture showing an apocrine secretion was opened to sight since the cells often protruded their own cytoplasmic processes faintly stained with iron hematoxylin. In the constant oestrus, the epithelial cells demonstrated the similar pattern either to prooestrus and oestrus or to a castration response, although they were even lower than those in above two oestrus stages, and did not perform an apocrine secretion, as the luminal surface was plane. When progesterone was administered to the castrated rats, the effect of castration was kept in the epithelial cells whose cytological feature was consistent with metoestrus.<BR>Concerning the changes of fat granules in the epithelial cells under various experimental conditions, it was informed that castration and constant dioestrus increase the number and size of them, while estrogen administration to the castrated rats induces remarkable decrease in number and progestrone cannot give an influence upon them. A decided conclusion was not able to be drawn as to the glycogen content (idntified by PAS reaction) in the epithelial cells following the estrogen and progesterone administrations. It was probable, however, that the action of progesterone to the precipitation of fat granules is superior in degree to that of estrogen.
- 日本内分泌学会の論文