歴代雌雄甲状腺機能欠如 (Radiothyroidectomized) Wistar系シロネズミの子孫 (F<SUB>1</SUB>-F<SUB>4</SUB>) に関する研究
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Choosing albino rats of the Wistar strain for study, the author destructed the thyroid glands of the rats of both sexes using radioactive iodine I<SUP>131</SUP>, crossed them above 1 month later and made them sire litters (F<SUB>1</SUB>). The destruction of thyroids of the young, both male and female, was achieved 3 months after birth in a similar manner that their parents were treated, mated above 1 month post-administration and made to give birth to the F<SUB>2</SUB> offsprings. The same procedure was conducted on four generations till the fourth descendants were obtained thereby, scheming to explore the influence of radiothyroidectomized state upon the descendants of successive four generations. On the offspring rats F<SUB>1</SUB> (26 cases), F<SUB>2</SUB> (26 cases), F<SUB>3</SUB> (29 cases) and F<SUB>4</SUB> (40 cases), each generation was subjected to the study of growth rate by determining body weights, to the comparative study of the weights of splanchnic organs at a definite time post partum, to the histological and histometrical investigations of thyroids and other splanchnic organs and was rendered to the measurements of their serum PBI, of serum total cholesterol and of the rate of thyroid uptake of I<SUP>131</SUP> and to the comparison with those of the control animals (25 cases), yielding the following results : <BR>(1) From the results of the measurement of body weights, it was revealed that the gain in body weights as compared to those of controls was reduced by degrees as the generation went down.<BR>(2) The ratio of thyroid-to body weights tended to increase as the generation descended.<BR>(3) In the picture of thyroid glands, follicles grew larger on and on, follicular cavities were filled with colloids, became to take up much eosin and PAS dye and the formation of peripheral vacuolation were depressed as the descent of generation went on.<BR>Follicle cells went on flattening, PAS granules seen in follicle cells were decreased in number parallel to the descent of generation, and the majority of follicle cells were found to present a similar appearance to that of endothelial cells.<BR>(4) From the histometrical examination of thyroids, the following results were obtained a) Follicle areas measured 5012±524μ<SUP>2</SUP> in controls, 5645±539μ<SUP>2</SUP> in F<SUB>1</SUB>, 6539±846μ<SUP>2</SUP> in F<SUB>2</SUB>, 7048±863μ<SUP>2</SUP> in F<SUB>3</SUB> and 7513±1048μ<SUP>2</SUP> in F<SUB>4</SUB>, tending to grow larger as the generation descended.<BR>b) Areas of follicle cavity were of the dimension of 2468±498 in controls, 3527±654μ<SUP>2</SUP> in F<SUB>1</SUB>, 4457±526μ<SUP>2</SUP> in F<SUB>2</SUB>, 5403±322μ<SUP>2</SUP> in F<SUB>3</SUB> and 6086±676μ<SUP>2</SUP> in F<SUB>4</SUB> which shows that the value of controls was about one half that of F<SUB>4</SUB>.<BR>c) Areas of follicle cells ranged to 129.3±16.7μ<SUP>2</SUP> in controls, 108.6±25.6μ<SUP>2</SUP> in F<SUB>1</SUB>, 96.1±21.2μ<SUP>2</SUP> in F<SUB>2</SUB>, 75.9±12.4μ<SUP>2</SUP> in F<SUB>3</SUB> and 66.0±11.7μ<SUP>2</SUP> in F<SUB>4</SUB>, which means the fall on successive generations.<BR>d) Follicle cells were of the height of 7.7±0.585μ in controls, 6.8±0.937μ in F<SUB>1</SUB>, 6.1±0.789μ in F<SUB>2</SUB>, 5.3±0.603μ in F<SUB>3</SUB> and 4.4±0.44μ in F<SUB>4</SUB>, showing a tendency to an apparent decrease on consecutive descent of generations.<BR>e) Cell counts per follicle cell amounted 19.9±2.53 in controls, 20.3±2.41 in F<SUB>1</SUB>, 20.3±3.96 in F<SUB>2</SUB>, 21.6±2.26 in F<SUB>3</SUB> and 20.7±2.53 in F<SUB>4</SUB>, minor changes being observed in cell counts.
- 一般社団法人 日本内分泌学会の論文