Pregnane-3α, 20α-diolの螢光定量法に関する基礎的研究
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概要
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The authors decided the optimal conditions for fluorometry of pregnanediol which was intended to be applied for measurement of β-glucuronidase activity.<BR>They measured the fluorescent spectra of pregnanediol reacted with concentrated sulfuric acid, antimonous trichloride and phosphoric acid, respectively.<BR>For fluorometry the dried residue of pregnanediol was treated with 3 ml of concentrated sulfuric acid for 60 minutes in 100°C water bath, then cooled in tap water and allowed to stand for 60 minutes at room temperature. The reaction of preganediol with sulfuric acid proceeded even at room temperature and became stabilized in 45 to 50 minutes. Then the intensity of fluorescence was determined by self-made fluorometer with the exciting light of 436 mμ. Pregnandiol in amounts ranging from 0.01 to 10 μg can be satisfactorily determined.
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