Silber-Porter法による血中17-Hydroxycorticosteroidの測定法ならびに本邦人末梢循環血液中の17-Hydroxycorticosteroid値について

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The present report concerns with the supplementary studies on Silber-Porter's method which is used to estimate adrenocortical function in both clinical and experimental fields as a simplified procedure.<BR>We reconfirmed the importance of purification of reagens, especially that of chloroform and phenylhydrazine, as has been emphasized by previous reporters.<BR>Particularly in purifing the latter, unglazed pottery plate to absorb solvent is considered to be worthy of extensive application.<BR>More clear-cut result were obtained by adding 8μgm. of standard hydrocortisone than those by the original method in which 5μgm. is appended.<BR>According to our modified Silber-Porter's method, the concentration of the cortical steroid was estimated from the peripheral circulating blood of Japanese under normal condition. The value estimated is statistically slightly lower than that of Anglo-Americans, but coincides fairy well with the reports of other investigation of Japanese. It is, therefore, concluded that the concentration of 17-21-dihydroxy-20-ketosteroid contained in blood of the Japanese is generally lower than that of Anglo-Americans.
日本内分泌学会の論文