内分泌臓器剔出法の研究:1. マウス腦下垂体剔出法 (小山氏外聴道法応用)
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概要
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We can successfully find a method of hypophysectomy on the mouse by the modification and improvement of Koyama's Method (external auditory canal method).<BR>Our method of hypophysectomy is to be conducted as fellow:<BR>1. The mouse is anesthetized with ether.<BR>2. The needle (1/1 injection needle) is used to fit with the injector being 2cc in size.<BR>3. The injection needle held as its cut surface downwards is inserted in the external auricular opening and after breaking through tympanic small canal run carefly 2mm verticaly to the longitudinal axis of body.<BR>4. Drawing gently the inside cylinder of injector and then hypophysis is intered into the injector.<BR>5. We can probe hypophysis with capillary tubesnet and distinguish its anterior-lobe from posteriorlobe in a drop of glycerine under microscope.<BR>6. The depth of perforation is 2mm plus to the half of distance between both incisura interlobularis.
- 一般社団法人 日本内分泌学会の論文