抗利尿物質に関する研究(第1報):測定法並びに正常人血漿抗利尿活性度
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As the assay methods for antidiuretic substance usually employed are neither sensitive nor accurate, I tried to find more precise method using the response on the antidiuretic activity in natural urine voiding of rats.<BR>I observed the natural urine voiding of adult rats hydrated with tap water of five per cent body weight at hourly intervals. Within 10-20 minutes after the second gavage of water the rats maintained diuresis in a constant rate and the constant diuresis persisted no less than 60 minutes. When the urine volume was measured 3 times or more until 40 minutes after the second gavage, the urine volume to be excreted for the subsequent 40 minutes could be expected.<BR>An apparatus for urine collection for indicating automatically the time of natural urine voiding of several rats was designed and the material to be tested was injected into the tail vein of non-anesthetized rats before 40 minutes after the second water administration. The injection dose was uniformly 0.1ml. per 100 grams rat body weight.<BR>Antidiuretic activity of the material was expressed as mean of the antidiuretic response (%) obtained from several rats:<BR>Antidiuretic Response<BR>A: The urine volume expected to be excreted for 40 minutes without injection of material.<BR>B The urine volume observed to be excreted for 40 minutes after injection of material.<BR>The effects of injections of normal saline into 15 rats were almost negligible and those of graded doses of pitressin into 50 rats showed a linear relationship between log? response (R) and dose (D) ranged 0.2-0.8 milliunit per ml.:<BR>Log. R=1.188+0.83 D (p<0.01)<BR>Assays for antidiuretic activity of the plasma of normal subjects were performed in 36 cases. The blood specimens were withdrawn from 12 normal subjects in the early morning, in the midday and in the evening, before each meal respectively. The results obtained revealed that plasma antidiuretic activity in the normal subjects was quite slight in the early morning, while significantly increased in the midday and tended to decrease in the evening.
- 一般社団法人 日本内分泌学会の論文