血清抗利尿物質ADSの本態に関する実驗的臨床的検討
スポンサーリンク
概要
- 論文の詳細を見る
It is considered that the bioassay of serum antidiuretic substances (ADS) measures the combined effect of the neurohypophyseal antidiuretic hormone (ADH) and other antidiuretic factors (ADF).<BR>In the bioassay of ADS using intact rats, antidiuretic action on the test animals is produced not only by ADH but also by other antidiuretic factors and the result does not show the value of ADH itself.<BR>In our study on the bioassay of serum ADS in patients and animals using Birnie's method, it was strange that serum ADS in some patients with Diabetes insipidus does not show a low value. It has not been known whether or not, the increase of serum ADS in the bilaterally adrenarectomized rats is due to the increase of secretion of ADH and whether or not the increase of serum ADS in the hypophysectomized rats, 30 days after operation and in the hypophysecto-adrenarectomized rats are due to the compensated secretion of ADH from the hypothalamus!<BR>It was assumed that the bioassay of serum ADS using the hypophysectomized rats was able to measure ADH itself. Hypophysectomized rats showed constant and sensitive antidiuretic reaction to the injection of the low unit of pitressin (Parke Davis). By this method of bioassay a certain amount of ADH was detected in the serum of normal subjects but no ADH was detected in the serum of patients with Diabetes insipidus. This result suggests the existent of the compensated increase of ADF in Diabetes insipidus. The value of serum ADH in the adrenarectomized rats were similar to the value in the normal rats, the value in the hypophysectomized rats, 30 days after operation, were zero and the value in the hypophysecto-adrenarectomized rats were almost zero.<BR>From these observations, it has been shown that the bioassay of ADH with intact rat is influenced by the antidiuretic factors other than ADH. Therefore, hypophysectomized rat should be used for the bioassay of ADH.
- 一般社団法人 日本内分泌学会の論文