ちんめるまん反應ノ應用
スポンサーリンク
概要
- 論文の詳細を見る
The urinary 17-ketosteroid excretions were assayed with the ZIMMERMANN's m-dinitrobenzene reaction by a rapid micromethod, modified those of DREKTER et al (1947) and PEARSON et al (1948). As the colorimetric assay procedures were applied to crude neutral urine extracts, the color correction equation of FRASER et al (1941) was used for eliminating the error of overe stimation of ketonic steroids, contributed by other interfering chromogens. The standard crystalline steroids used were ketonic androsterone, dehydroandrosterone, and nonketonic testosterone.<BR>Various precedures introduced by preceding authors for extraction, fractionation, purification, and color formation of the 17-ketosteroids widely reviewed, and clinical availabilities of those were discussed.<BR>The normal values of the 17-ketosteroids excretion of the Japanese people were examined on 66 male and female subjects of various age groups. Average daily excretion of 17-ketosteroids for children under 5 and those 5-12 were 1.33 and 1.9mg., respectively. For males of age groups of 13-16, 17-34, and 35-49, the values were 3.4, 11.9, and 11.0mg., respectively. The second age group was subdivided intö four sections-17-19, 20-24, 25-29, and 30-34. For these four groups of the males the corresponding values were 9.2 11,6, 13.4, and 13.6mg. Average excretion for females of 13-16 and those 17-53 were 3.8 and 9.3mg., respectively. All of the examined 66 subjects were exclusively sound and had no clinical evidence of either endocrine or nonendocrine disorders.
- 日本内分泌学会の論文