タンパク質の翻訳後修飾の構造生物学研究

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概要

• 論文の詳細を見る

•   A majority of proteins encoded in genomes of limited size are post-translationally diversified by covalent modifications such as glycosylation and ubiquitination. Although recent advances in structural proteomics have enabled high-throughput structure determination of proteins, structural analyses of post-translationally modified proteins remain challenging because of the lack of appropriate determination methods. Therefore, we developed methodologies for characterizing the post-translational modifications of proteins from the structural viewpoint, focusing especially on glycosylation and ubiquitination. For instance, we established a systematic method for structural glycomics to address broader issues, including glycosylation profiling and 3D structure analyses of glycoproteins. Our stable-isotope-assisted NMR techniques in conjunction with X-ray crystallographic approach provide valuable information at the atomic level on conformations, dynamics, and interactions of glycoproteins such as antibody and proteins involved in the ubiquitin-proteasome system. These studies provide the structural basis for improved efficacy of therapeutic antibodies on defucosylation of their Fc glycans and mechanistic insights into ubiquitination reactions in glycoprotein-fate determination in cells. These approaches will allow new possibilities for structural studies on post-translationally modified proteins of clinical, pathological, and pharmaceutical interests.

• 公益社団法人　日本薬学会の論文

著者

• 加藤 晃一

  自然科学研究機構 岡崎統合バイオサイエンスセンター

• 加藤 晃一

  自然科学研究機構岡崎統合バイオサイエンスセンター/分子科学研究所

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• 細胞の中の不要なタンパク質に目印をつける仕組み
• 2SG-01 ユビキチン-プロテアソームシステムにおけるオリゴマータンパク質の構造揺らぎ(2SG 生命分子の揺らぎを探る新たなアプローチ,日本生物物理学会第49回年会(2011年度))
• タンパク質の翻訳後修飾の構造生物学研究

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