Construction of Swine-Specific CpG Motif Plasmid and the Study of Its Immunostimulatory Effects both In Vitro and In Vivo

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概要

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• A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed high Stimulation Index (SI) compared to the positive control (LPS). In a following animal vaccination-challenge experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period.

著者

• Sanchez-vizcaino Jose

  Centro De Investigacion En Sanidad Animal Inia Ctra. Valdeolmos A El Casar Sn

• Zhang Quan

  College Of Life Sciences Peking University

• ZHU Gang

  Xinjiang Tiankang Animal Science Bio-Technology Co. Ltd., Urumqi, 830000, China

• SUN Yao

  China Animal Husbandry Group Research Institute, Beijing 100081, China

• SÁNCHEZ-VIZCAÍNO José

  Centro de Vigilancia Sanitaria Veterinaria (VISAVET) and Animal Health Department, Complutense University of Madrid, Avda. Puerta de Hierro, S/N 28040 Madrid, Spain

• GUO Xiaoyu

  Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

• JIA Hong

  Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

• YUAN Weifeng

  Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

• HOU Shaohua

  Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

• ZHU Hongfei

  Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

関連論文

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• Examination of the Cytoplasmic DNA in Male Reproductive Cells to Determine the Potential for Cytoplasmic Inheritance in 295 Angiosperm Species
• Construction of Swine-Specific CpG Motif Plasmid and the Study of Its Immunostimulatory Effects both In Vitro and In Vivo

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