A Case Report in Male Gamete Rescued from a Siamese Eld’s Deer (Rucervus eldi siamensis): Post-Thawed Testicular, Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability

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In the present study, quality of frozen-thawed epididymal and testicular sperm recovered from a Siamese Eld’s deer was examined. The epididymal sperm quality was assessed in fresh, cold-stored at 4°C and frozen-thawed samples. Zona binding ability of the frozen-thawed epididymal samples with Burmese Eld’s deer oocytes were also evaluated. Testicular sperm extracted from tissues frozen at −80 or −196°C for one month were examined for membrane and DNA integrity. Epididymal sperm retained their quality up to 24 hr of cold stored at 4°C. The frozen and thawed epididymal sperm showed 30, 46.5, 27 and 89.5% in sperm motility, intact membrane, acrosome and DNA and had ability to bind with oocytes with the average number of 92.5 ± 64 sperm/oocytes. The sperm extracted from testicular tissues cryopreserved at −196 and −80°C for one month showed around 70% intact membrane. In conclusion, epididymal and testicular sperm are reported survived after 13 hr post-mortem. Furthermore, cold storage at 4°C and cryopreservation at −196 and −80°C maintain the quality of epididymal and testicular sperm. This study represents a model for male gamete rescue in endangered Eld’s deer.