CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications
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概要
- 論文の詳細を見る
Fluorescence in situ hybridization (FISH) has become a standard technique in environmental microbiology. More than 20 years have passed since this technique was first described, and it is currently used for the detection of ribosomal RNA, messenger RNA, and functional genes encoded on chromosomes. This review focuses on the advancement and applications of FISH combined with catalyzed reporter deposition (CARD, also known as tyramide signal amplification or TSA), in the detection of environmental microorganisms. Significant methodological improvements have been made in CARD-FISH technology, including its combination with other techniques and instruments.
著者
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Kubota Kengo
Department Of Civil And Environmental Engineering Tohoku University
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Kubota Kengo
Department of Civil and Environmental Engineering, Tohoku University
関連論文
- Detection of Single Copy Genes by Two-Pass Tyramide Signal Amplification Fluorescence in situ Hybridization (Two-Pass TSA-FISH) with Single Oligonucleotide Probes
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- PB-29 In situ visualization of single copy genes at the cellular level by ultra sensitive two-pass TSA-FISH with oligonucleotide probes(Monitoring,Poster presentation B)
- PB-16 Microbial community shift during cultivating a inoculum for mesophilic anaerobic digestion from waste activated sludge and cattle manure(Waste water treatment ecosystem,Poster presentation B)
- CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications