2′-Benzoyloxycinnamaldehyde-Mediated DJ-1 Upregulation Protects MCF-7 Cells from Mitochondrial Damage
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概要
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2′-Benzoyloxycinnamaldehyde (BCA) is a promising antitumor agent which induces cancer cells apoptosis via reactive oxygen species (ROS) generation. BCA shows more effective antiproliferation in MDA-MB-435 than in MCF-7 breast cancer cells. DJ-1 has been known to protect cells against oxidative stress as an antioxidant because of its cysteine residues sensitive to oxidative stress. In the present study, we evaluated the mechanism of DJ-1 for cell protection from oxidative stress after BCA treatment in MCF-7 cell. BCA upregulates the expression of DJ-1 in MCF-7 cells. However, DJ-1 expression decreased continuously for 24 h after BCA treatment in MDA-MB-435 cells. DJ-1 knockdown sensitized MCF-7 cells to BCA, on the contrary, DJ-1 overexpression induced MDA-MB-435 cells less sensitive to BCA. Confocal microscopic observation showed that only in MCF-7 cells BCA increased the overlapped signal between mitochondria and DJ-1 protein. Mitochondrial membrane potential (MMP) was decreased in MDA-MB-435 cells by BCA, and DJ-1 overexpression inhibited BCA-induced MMP decrease in these cells. On the contrary, DJ-1 knockdown in MCF-7 induced MMP perturbation by BCA. These findings suggest that DJ-1 upregulation protects MCF-7 cells from BCA via inhibiting mitochondrial damage.
著者
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Kang Hye
Department Of Internal Medicine Korea Cancer Center Hospital
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Kwon Byoung-mog
Laboratory Of Chemical Biology And Genomics Korea Research Institute Of Bioscience And Biotechnology
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Hong Su-hyung
Department Of Dental Microbiology School Of Dentistry Kyungpook National University
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Lee Heon-jin
Department Of Dental Microbiology School Of Dentistry Kyungpook National University
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Lee Hae-ahm
Department Of Dental Microbiology School Of Dentistry Kyungpook National University
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Ismail Ismail
Department Of Dental Microbiology School Of Dentistry Kyungpook National University
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Hong Su-Hyung
Department of Oral Microbiology, School of Dentistry, Kyungpook National University
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Kwon Byoung-Mog
Laboratory of Chemical Biology and Genomics, Korea Research Institute of Bioscience and Biotechnology
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Ismail Ismail
Department of Oral Microbiology, School of Dentistry, Kyungpook National University
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Kang Hye
Department of Oral Microbiology, School of Dentistry, Kyungpook National University
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KANG Hye
Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University
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