Multidrug Resistance Associated Protein 1 together with Glutathione Plays a Protective Role against 4-Hydroxy-2-nonenal-Induced Oxidative Stress in Bovine Aortic Endothelial Cells
スポンサーリンク
概要
- 論文の詳細を見る
4-Hydroxy-2-nonenal (HNE), an aldehyde produced by lipid peroxidation, induces cytotoxicity and oxidative stress. Glutathione (GSH) protects against the cytotoxicity of HNE. However, the protective mechanism of GSH has not been fully examined. We examined the protective role played by the relationship between GSH and multidrug resistance associated protein 1 (MRP1) against the HNE-induced oxidative stress in bovine aortic endothelial cells (BAECs). HNE induced the loss of viability of BAECs. Exogenous GSH, which is membrane-impermeable, prevented the loss of viability induced by HNE by inhibiting HNE uptake in BAECs, probably due to the formation of the HNE-SG complex in the extracellular space. We demonstrated that HNE induced the expression of MRP1 protein, which can transport the HNE-SG complex. The induction of MRP1 protein expression by HNE disappeared in BAECs pretreated with L-buthionine sulfoximine, a GSH-depleting agent. This result suggests that HNE, together with intracellular GSH, contributes to the regulation of MRP1 protein expression. Moreover, we found that MK571, an MRP1 inhibitor, promoted the HNE-induced oxidative stress and cell death. Taken together, these findings suggest that MRP1, together with GSH, plays a protective role against the HNE-induced oxidative stress in BAECs.
著者
-
Takahashi Kyohei
School of Pharmacy, Hokkaido Pharmaceutical University
-
Tatsunami Ryosuke
School of Pharmacy, Hokkaido Pharmaceutical University
-
Tampo Yoshiko
School Of Pharmacy Hokkaido Pharmaceutical Univ.
-
Sato Keisuke
School of Pharmacy, Hokkaido Pharmaceutical University
関連論文
- Buthionine Sulfoximine Promotes Methylglyoxal-Induced Apoptotic Cell Death and Oxidative Stress in Endothelial Cells
- Multidrug Resistance Associated Protein 1 together with Glutathione Plays a Protective Role against 4-Hydroxy-2-nonenal-Induced Oxidative Stress in Bovine Aortic Endothelial Cells