Alteration of Photosynthate Partitioning by High-Level Expression of Phosphoglucomutase in Tobacco Chloroplasts
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概要
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Plastidial phosphoglucomutase (PGM) plays an important role in starch synthesis and degradation. Nonetheless, the impact of enhanced plastidial PGM activity on metabolism in photosynthetic tissue is yet to be elucidated. In this study, we generated transplastomic tobacco plants overproducing Arabidopsis thaliana plastidial PGM (AtptPGM) in chloroplasts and analyzed the consequent metabolic and physiological parameters in the transplastomic plants. AtptPGM accumulated in the chloroplasts to up to 16% of total soluble protein in the leaves. PGM activity in leaves increased 100-fold relative to that of wild-type plants. The transplastomic plants were phenotypically indistinguishable in their growth rates, photosynthetic activities, and starch synthesis from wild-type plants, but hexose partitioning in the light period was dramatically different. Furthermore, alteration of extracellular invertase activity was observed in the lower leaves of the transplastomic plants. These observations suggest that high-level expression of plastidial PGM alters hexose partitioning in light periods via modification of extracellular invertase activity.
- Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文
著者
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INUI Masayuki
Research Institute of Innovative Technology for the Earth (RITE)
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Suzuki Nobuaki
Research and Education Center For Genetic Information
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Inui Masayuki
Research Institute Of Innovative Technology For The Earth
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Yukawa Hideaki
Research Institute Of Innovative Technology For The Earth
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Suzuki Nobuaki
Research Institute Of Innovative Technology For The Earth (rite)
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Uematsu Kimio
Research Institute Of Innovative Technology For The Earth (rite)
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IWAMAE Tomoko
Research Institute of Innovative Technology for the Earth (RITE)
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- Alteration of Photosynthate Partitioning by High-Level Expression of Phosphoglucomutase in Tobacco Chloroplasts
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