Extraction of High-Quality RNA from Rubber Tree Leaves
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概要
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A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.
著者
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DENG Liu-Hong
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
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LUO Ming-Wu
College of Materials Science and Chemical Engineering, Hainan University
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ZHANG Chun-Fa
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
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ZENG Hui-Cai
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
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Luo Ming-wu
College Of Materials Science And Chemical Engineering Hainan University
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Zeng Hui-cai
Institute Of Tropical Bioscience And Biotechnology Chinese Academy Of Tropical Agricultural Sciences
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Zhang Chun-fa
Institute Of Tropical Bioscience And Biotechnology Chinese Academy Of Tropical Agricultural Sciences
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Deng Liu-hong
Institute Of Tropical Bioscience And Biotechnology Chinese Academy Of Tropical Agricultural Sciences
関連論文
- Extraction of High-Quality RNA from Rubber Tree Leaves
- Extraction of High-Quality RNA from Rubber Tree Leaves