Interrelationships Between Apoptosis and Fertility in Bull Sperm

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概要

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• Male fertility, the ability of sperm to fertilize and activate the egg and support early embryogenesis, is vital for mammalian reproduction. Despite producing adequate numbers of sperm with normal motility and morphology, some males suffer from low fertility whose molecular mechanisms are not known. The objective was to determine apoptosis in sperm from high and low fertility bulls and its relationship with male fertility. DNA damage, phosphatidylserine (PS) translocation, and expression of pro- and anti-apoptotic proteins (BAX and BCL-2) in the sperm were determined using TUNEL, Annexin V, and immunoblotting approaches, respectively. Amounts of apoptotic spermatozoa were 2.86 (±1.31) and 3.00 (±0.96) in high and low fertility bulls, respectively (P=0.548), and were not correlated with fertility. There was a negative correlation between early necrotic spermatozoa and viable spermatozoa (r = −0.99, P<0.0001). Fertility scores were correlated with live spermatozoa detected by eosin-nigrosin test and necrotic spermatozoa determined via flow cytometry (r = −0.49, P<0.006 and r = −0.266, P<0.0113, respectively). BAX level was higher in low fertile group than high fertile group; however, this difference was not statistically significant due to the variations of bull samples (Bull 1-3 vs. Bull 4-5) in low fertile group (P<0.283). BCL-2 was not detectable in any of the sperm samples. The results shed light onto molecular and cellular underpinnings of male fertility.

著者

• Memili Erdogan

  Department Of Animal And Dairy Sciences Mississippi State University

• BELSER Lauren

  Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS 39762, USA

• DOGAN Sule

  Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS 39762, USA

• MASON Melissa

  Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS 39762, USA

• GOVINDARAJU Aruna

  Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS 39762, USA

• KAYA Abdullah

  Alta Genetics, Incorporated, Watertown, WI 53094, USA

• STOKES John

  Department of Basic Sciences, Mississippi State University, Starkville, MS 39762, USA

• ROWE Dennis

  Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Starkville, MS 39762, USA

関連論文

• Interrelationships Between Apoptosis and Fertility in Bull Sperm
• Mycotoxin Alpha-Zearalenol Impairs the Quality of Preimplantation Porcine Embryos
• Interrelationships Between Apoptosis and Fertility in Bull Sperm

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