Synchronization Using PGF2α and Estradiol with or without GnRH for Timed AI in Dairy Cows
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概要
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This study examined the use of PGF2α and estradiol benzoate (EB) either with or without GnRH to synchronize estrus in dairy cows for timed artificial insemination (TAI) under field conditions. First, Holstein dairy cows with a corpus luteum (CL) received 500 μg cloprostenol and were then randomly allocated to three groups: no further treatment (control, n = 236); treatment with 1 mg EB 56 h after cloprostenol (EB group, n = 339); or treatment with 1 mg EB 56 h after cloprostenol followed by treatment with 100 μg gonadorelin 24 h later (EB + GnRH group, n = 216). All cows received TAI 80 h after the cloprostenol injection. In a second experiment, Holstein dairy cows with a CL received 500 μg cloprostenol and were then randomly allocated to two groups: treatment with 2 mg EB 36 h later (EB group, n = 284) or treatment with 2 mg EB 36 h after cloprostenol followed by 100 μg gonadorelin 24 h later (EB + GnRH group, n = 229). All cows received TAI 24 h after the EB injection. Logistic analyses revealed that the odds ratio for the probability of pregnancy when 1 mg EB was administered 56 h following cloprostenol was 1.9 and 2.0 times (P < 0.001) higher in the EB (39.5%) and EB+GnRH groups (40.7%), respectively, compared with the control group (25.8%). However, pregnancy rates in cows receiving 2 mg EB 24 h following cloprostenol showed no difference compared with cows treated with EB only (32.4%) or with EB+GnRH (35.8%). These results indicate that a synchronization protocol comprising PGF2α and EB could be used for TAI in dairy herds under field conditions.
著者
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KANG Hyun
College of Life Sciences and Biotechnology, Korea University
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KANG Hyun
College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, South Korea
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JEONG Jae
College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, South Korea
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HUR Tai
National Institute of Animal Science, RDA, Cheonan 330-801, South Korea
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KIM Ill
College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, South Korea
関連論文
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