Reserpine投与による実験胃潰瘍の発生に関する研究--特に,ラット脳内のMAO活性値およびserotonin量の変動について
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Experimental gastric ulcer in rat was induced artificially by the administration of reserpine (i.p.), and change in MAO activity and serotonin level in brain were studied simultaneously. In addition, fluorescence histochemical analysis of gastric tissue was also studied. The following results were obtained :<BR>1.<I>In vitro</I>, reserpine increased MAO activity in brain, and which effect was proportional to the reserpine concentration tested.<BR>2.<I>In vivo</I>, reserpine also increased the MAO activity in brain. At three hours after the administration of reserpine, MAO activity in brain showed its maximum and it was 149% of the control value. On the other hand, at this stage, serotonin level in brain decreased markedly, and it was 35% of the control value and a slight gastric hemorrhage was observed. A few hemorrhagic erosions were observed in the gastric mucosa at 24 hours after the administration. The administration of iproniazid caused decrease in MAO activity in brain, but serotonin level in brain showed a marked increase and reached maximum of 187% of the control value at 6 hours after the adminstration. When reserpine was administered 15 minutes following the administration of iproniazid, MAO activity in brain decreased markedly and it showed the lowest activity at 30 minutes and then it tended to return to its control level. The gastric mucosa was examined at 3 and 24 hours after the administration of iproniazid, but no gross change were detected. On the other hand, the serotonin level reached its maximum and it showed 162% of the control value at 1 hour after the administration. A gross examination of the gastric mucosa clearly revealed numerous hemorrhagic erosions.<BR>3. At 15 minutes following the administration of reserpine, wide spread yellow fluorescence gastric tissue was observed in fluorescence histochemical analysis. This was the image of serotonin and adrenergic amines released by reserpine from granule cells in gastric tissue. In gastric tissue at 30 minutes to 3 hours after the administration, fluorescence of the adrenergic amines completely disappeared and only the yellow fluorescence of the serotonin could be detected in the glandular ducts. But at 24 hours after the administration almost no difference could be detected in comparison with that of the control.
- 昭和大学・昭和医学会の論文