体液中抗生物質の微量測定法--特に小児の全血について
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概要
- 論文の詳細を見る
Many determination methods of antibiotics concentration in the body fluid are available, but there are a few simple micromethod for determination, especially in the cases of premature, newborn and young children.<BR>The auther diviced a vertical diffusion method using agar medium in tube. The material isnstilled in gauze was put on the medium and the procedure was carried out according to Torii-Kawakami method. The materials were phosphate buffer, serum and difibrirated blood that contain antibiotics.<BR>In the examination in vitro, the result with Amino-benzyl Penicillin (Ab-Pc), Cepha-loridin (CER), Acetyl-Spiraymycin (Ac-SPM), Lincomycin (LCM), Tetracyclin (TC) and Chloramphenicol (CPL) corresponded completely that with Torii-Kawakami method. The effect of serum and blood cell on the drugs was different from each drugs. Especially, inhibitory zone by putting blood cells on the medium became shorter than that by putting serum. The standard curve of the former was narrowed than that of latter.<BR>In clinical medication with Ab-PC, CER, Ac-SPM and LCM, the result was corresponded with Torii-Kawakami method.<BR>The inhibitory zone using whole blood was shorter like the examination in vitro. The active concentration of whole blood which was determined with standard curve using human defibrirated blood was hardly different from that using serum. Because of difficulty of getting human defibriated blood, defibriated blood of horse was used. In the examination with Ab-PC, CER and LCM, no defference between the inhibitory zone by defibrirated blood of human and horse was found.<BR>In conclusin, auther's micromethod of determination was seemed to be usef ull as a laboratory examination for antibiotics concentration in the cases of premature, newborn and young children, because of little difficulty of technique and the small amount of the meterial.
- 昭和大学・昭和医学会の論文