Diphtheria菌迅速同定法について
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概要
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The identification of C. diphtheriae in clinics is liable to depend only on the morphological findings in the stained smears of pharyngeal secretion, especially white membrane, because of the long time necessary for the complete identification of this organism.The authors therefore tried to shorten the length of timc necessary for test. It was done for 291 children, from pharyngeas of whom 188 strains of Corynebacterium were isolated and 8 strains out of them were found to be C. diphtheriae.The organism could not be found in two third of cases when microscopic investigation was asked from general practitioners in the city, though C. pseudodiphtheriticum cnuld be found in almost all cases.The author's method was as follows;Zinnemann's unheated blood tellurite medium, D. S. S. agar and Schroer's method for the biological test were tries together and found to be useful in the diagnosis of this organism within 48 hours. Wohlfeil's urease test was effective in removing C. pseudodiphtheriticum out of specimens. Proteose peptone was in-dispensable for D. S. S. agar, because other peptones were found to be unsuitable.Each species of Corynebacterium showed similar sensitivity against 9 antibiotics and chemotherapeutics. Erythromycin was found to be most effective.
- 耳鼻咽喉科臨床学会の論文