THE RELATION OF ACTIVITIES OF REDUCED DIPHOSPHOPYRIDINE NUCLEOTIDE-DIAPHORASE AND SUCCINIC DEHYDROGENASE TO MITOSIS IN EHRLICH'S ASCITES TUMOR CELLS

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The succinic dehydrogenase and DPNH-diaphorase activities of Ehrlich's ascites tumor cells were studied histochemically and the changes in the nuclei were followed with a phase contrast microscope and the following results were obtained.1. Succinic dehydrogenase activity is very weak.2. Strong DPNH-diaphorase activity is found in about 10% of the cells but no activity was detected in the majority of them.3. In the cells showing no DPNH-diaphorase activity, the nuclear membrane and nucleoli were clearly observed and the cells were in the resting state, whereas in the active cells the nuclear membranes and nucleoli were absent, the chromosomes being coarse and diffusive, sometimes partially gathering in groups and being arranged. The cells were in the mitotic stage.4. The DPNH-diaphorase activity appears in a part of the cytoplasm, spreads throughout the whole cell reaching a maximum, then gradually weakens and eventually disappears. When the activity appears in a part of the cytoplasm, the nuclear membranes and nucleoli are clearly visible. Thereafter the cells are changed into pro- (highest activity), meta-, ana- and telophase and finally divided into daughter cells.5. Thus the DPNH-diaphorase is concluded to be closely related to mitosis.
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