ACTION OF S-BENZOYLTHIAMINE MONOPHOSPHATE ON YEAST

概要

論文の詳細を見る
1. The effect of S-benzoylthiamine monophosphate (BTMP) on yeast cells was investigated. The compound is known to have a superior affinity to animal tissues and to keep higher blood levels in animal body than thiamine. BTMP showed the thiamine activity on the growth and fermentation of the yeast, but the appearance of its effect was somewhat delayed as compared with thiamine.Considering from the ineffectiveness of BTMP on lactobacilli, a significant difference on absorbability is supposed between animal tissues and microorganism cell walls.2. In order to compare exactly the rate of the appearance of the activity of the two compounds, the fermentation test for thiamine assay of Schultz, Atkin and Frey was employed. BTMP was shown to behave similarly to SBT. The appearance of its effect was delayed by a quarter to one hour as compared with that of thiamine, but after the appearance the derivative showed the same accerelating effect on the growth of the yeast as thiamine. In the case of thiamine monophosphate (TMP) the retardation of the appearance of the effect was scarcely noticed. It is therefore assumed that BTMP is incorporated into the cells as TMP after debenzoylation. O-Benzoylthiamine disulfide showed the retardation of 5-6 hours before the appearance of the effect. It is therefore assumed that it takes much time for the production of the adaptive enzyme for cleaving the O-benzoyl group.3. The investigation of the forms of thiamine in the yeast cells incubated with BTMP by both chemical assay and paper chromatography showed the absence of acylated thiamine (BTMP and SBT), but more esterified thiamine was detected than those incubated with thiamine. It was found to be largely TMP. Thus it was clarified that BTMP is incorporated into the cells as TMP after debenzoylation.
日本ビタミン学会の論文

ACTION OF S-BENZOYLTHIAMINE MONOPHOSPHATE ON YEAST

スポンサーリンク

概要

著者

関連論文

スポンサーリンク