STUDIES ON THE EXISTING STATE OF VITAMIN A IN FISH LIVER AND OTHER ORGANS:II. CHEMICAL VERIFICATION FOR ADSORBABILITY OF VITAMIN A TO MELANIN PIGMENTS IN THE BLACK LIVER OF THE DOGFISH
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1. Using crude melanin separated from human hair, vitamin A was proved to be adsorbed fairly well on the melanin. The adsorbed vitamin could be liberated from melanin with ethyl ether without suffering oxidation or destruction.2. The same experiments were also carried out with the liver melanin isolated from the black-colored liver of the dogfish as well as with active carbon, and similar results were obtained.3. The adsorbability of vitamin A on active carbon, hair melanin, casein, liver protein and other substances in petroleum ether or in aqueous solution (solubilized by Tween 80) was compaired. The following order of adsorbability was obtained in both solvents:Active carbon>hair melanin, liver melanin>casein, liver protein, gelatin>powdered agar-agar, glass powder, defatted cotton wool.4. The order of adsorbability of natural vitamin A liver oil containing glyceride on melanin or other substances in petroleum ether was fairly the same as in the case of the vitamin alone, though the adsorbability was somewhat decreased.
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