SEPARATION AND DETERMINATION OF TOCOPHEROLS BY GAS CHROMATOGRAPHY

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概要

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• With the use of a highly sensitive gas chromatograph equipped with hydrogen ionization detector, separation and determination of α-, γ- and δ-tocopherols were carried out. Following acetylation of the analogues, they were injected into the gas chromatograph in amounts of 5μl of 1-3% acetone solution after addition of squalene as the internal standard. Peak area was determined with a half value width methed. A rectilinear relationship between the peak area and weight was obtained in α-, γ- and δ-compounds within the range of 20-100μg. Recovery was 96.0-103.0%. Separation and determination of tocopherol analogues contained in the natural concentrate were easily carried out.Although colorimetry according to the Nationnl Formulary gave similar values of total vitamin, higher values of α-tocopherol were always obtained with colorimetry than those determined by gas chromatography, probably indicating a defect in the determination of non-α-compound in colorimetry.

• 日本ビタミン学会の論文

著者

• 勝井 五一郎

  Research and Development Division, Eisai Co., Ltd

• 石川 靖二

  Research Laboratories of Eisai Co., Ltd.

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• SEPARATION AND DETERMINATION OF TOCOPHEROLS BY GAS CHROMATOGRAPHY
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