Effect of Dimethyl Sulphoxide on Charge Transfer Complexes between Serotonin-, Tryptophan and Flavin Mononucleotide under Low Temperatures
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The colors of serotonin-FMN and tryptophan-FMN complexes are not changed upon addition of dimethyl sulphoxide at the concentration of 10%, which is routinely used for cell preservation, at 20°. However, the addition to the extent of 50% and above does affect the colors of these complexes. The detailed measurement of the differences in the absorption spectra suggested that dimethyl sulphoxide weakens both the serotonin-FMN and tryptophan-FMN interactions at 20°. In contrast with the observation at 20°, the addition of DMSO under freezing conditions results in the disappearance of colors of the charge transfer complexes while raising the temperature to 20° promptly restores the original color. This disappearance and reappearance of colors, accompanied by freezing and thawing, could be repeated several times.
- 日本ビタミン学会の論文
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関連論文
- Effect of Dimethyl Sulphoxide on Charge Transfer Complexes of D-Amino Acid Oxidase under Low Temperatures
- Effect of Dimethyl Sulphoxide on Charge Transfer Complexes between Serotonin-, Tryptophan and Flavin Mononucleotide under Low Temperatures