Vitamin D Dependent Calcium Binding Protein in Rat Intestinal Mucosa

概要

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The purification and properties of vitamin D dependent calcium binding proteins were studied in rat intestinal mucosa. Two different vitamin D dependent calcium binding proteins were fractionated by the DEAE-cellulose column chromatography. Their properties were analyzed with the polyacrylamide gel disc electrophoresis and the sucrose density gradient ultracentrifugation. One of them is having a molecular weight about 24, 000 representing rapid migration on acrylamide gel electrophoresis and seems similar to the calcium binding protein obtained from chick intestinal mucosa in respect of its size and electrophoretic mobility. The other protein is a larger molecule (mol. wt. 145, 000) which is eluted in first fraction from DEAE cellulose chromatography. The smaller molecule of binding protein associates with one atom of calcium per one molecule of protein and the larger molecule would associate with more than 2 atoms of calcium.
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