Studies on Hog Intrinsic Factor:II. Immunoradioautographic Studies of the Properties of Hog Intrinsic Factor and Evidences Suggesting the Possible Existence in Hog Tissues of Hog Intrinsic Factor

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Using Ouchterlony's agar gel double diffusion and radioautography, the physicochemical properties of hog intrinsic factor and the probabilities of existence of hog intrinsic factor-like substance in various hog organs, such as liver, spleen, bile, serum, etc. were investigated.1. Hog intrinsic factor (IF) has resistance to 8 M urea and papain treatment in a bound or unbound form, and to 45% acetone and to pepsin treatment, if it was bound to B12.2. Hog IF was a little resistant to Nagase treatment, and heating to 100° within 30 minutes. It was completely destroyed by HCI treatment for 24 hours, pepsin treatment in a unbound form, α-chymotrypsin and trypsin in a relatively high concentration, 45% ethyl alcohol in both forms, and 45% acetone in an unbound form.3. By immunoautoradiography hog IF-like substance was found in the hog bile, serum, spleen, liver, but not in the kidney and thyroid gland. It suggests that hog IF-like substance might be contained in these organs.
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