Unusual Growth and Metabolism of the Biotin-Deficient Bacillus macerans
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概要
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Bacillus macerans required biotin as a growth factor. The effect of the addition of Tween 80, aspartate, arginine, uracil and AMP (or adenine), singly or collectively, to the biotin-deficient medium on the growth of cells was studied. In the experiments of single additions, only Tween 80 among these compounds partially replaced the action of biotin. The other compounds also showed a nonspecific biotin-replacing activity when they were added collectively to the medium.The accumulation of keto acids (mostly pyruvic acid) was found in the fluid of biotin-deficient culture. The amount of accumulation was roughly in inverse proportion to the cell growth.In the normal medium, cells grew better under relatively aerobic condition than under relatively anaerobic one. In the biotin-deficient medium, on the other hand, the relatively anaerobic condition was preferable for the growth of cells.The phosphorylating activity of the cell-free preparation from cells grown in the normal medium was higher under aerobic condition than that under anaerobic one. In the case of the biotindeficient cells, the cell-free preparation showed a higher phosphorylating activity under anaerobic condition than that under aerobic condition.
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