Spectrophotometric Demonstration of Vitamin B12S-Formation by Nucleophilic Cleavage of Co-C Bond of Acetylcobalamin

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概要

• 論文の詳細を見る

• In order to obtain further informations on the mechanism of the decomposition of the cobalt-carbon bond of acetylcobalamin by nucleophilic agents, the reaction was carried out using an appropriate apparatus under either aerobic or anaerobic conditions.By time-course study of the spectral changes, direct spectrophotometric evidence was presented for the formation of vitamin B12S as the initial intermediate in these reactions. Formation of acetic acid was also detected in the case of alkaline cyanidedecomposition. These facts indicate that the cleavage of the cobalt-carbon bond of acetylcobalamin by alkaline cyanide is caused by OH- ion generated from the hydrolysis of the cyanide in a similar way as in the case of β-cyanoethylcobalamin. Discussions on the mechanism of the conversion of vitamin B12S into dicyanocobalamin were described.

• 日本ビタミン学会の論文

著者

• 福井 三郎

  Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, K

• 清水 祥一

  Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University

• 山田 良平

  Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University

• 梅谷 友信

  Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University

• 福井 三郎

  Laboratory of Industrial Biochemistry, Deparmtnt of Industrial Chemistry, Faculty of Engineering, Kyoto University

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• TRANSFER OF PYRIDOXAL 5'-PHOSPHATE FROM ALBUMIN-PYRIDOXAL 5'-PHOSPHATE COMPLEX TO APO-ASPARTATE AMINOTRANSFERASE
• Spectrophotometric Demonstration of Vitamin B12S-Formation by Nucleophilic Cleavage of Co-C Bond of Acetylcobalamin
• STUDIES ON THE OXIDATION PRODUCTS OF LIPOIC ACID

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