ON THE METABOLISM OF COENZYME B12:II. DISAPPEARANCE FROM BLOOD AND DISTRIBUTION IN TISSUES OF COENZYME B12 AND HYDROXOCOBALAMIN AND THE EFFECT OF INTRINSIC FACTOR ON THE DISTRIBUTION OF BOTH COBAMIDES IN TISSUES FOLLOWING

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1, The clearance in the blood and the distribution in tissues of coenzyme B12 were examined in comparison with cyanocabolamin in both cases of single and simultaneous administration.2, Coenzyme B12 disappeared faster from the blood and showed a stronger affinity for tissues than cyanocobalamin3. By differential counting method with medical spectrometer coenzyme B12 and cyanocobalamin did not show any competition when taken in tissues.4. In the case of vitamin B12 deficient rats the distribution in tissues of cyanocobalamin was not so different from normal rats, while that coenzyme B12 was enhanced. Thus the coenzyme seems to be one of the storage form of the vitamin in the liver.5. The coenzyme uptake in the vitamin suturated rats was reduced, suggesting the conversion of the vitamin into the coenzyme.6. In case of an intravenous injection hydroxocobalamin showed a faster disappearance from the blood and a slower urinary excretion than cyanocobalamin, showing that hydroxocobalamin has a strong affinity for tissues. It was actually verified with a biological test.7. Intrinc factor was found to have some influence on the hepatic uptake of the coenzyme, which showed a remarkable increase in a short time.
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