An Experimental Study on Alkaline Phasphatase
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Activities and some behaeviors of the serum alkaline phosphatase (AP) and of the bone and liver extract AP, which were prepared from experimental rachitic rats, 3′-Me-DAB rats and normal rats, were examined in this paper.Results were as followes:1. In the rachitic rats sera AP activities, the maximum intensity was attained at sixth week of life prior to the radiological appearances of the rachitic changes.2. In the inhibitory reaction of the 0.05mM EDTA, the bone AP was inhibited about 50% but the rachitic sera was not inhibited so much at the same concentration EDTA, and then, at the 5mM EDTA, the behaeviors of the rachitic sera AP were different from those of the 3′-Me-DAB sera AP.3. At the high concentration Urea, the AP of the rachitic sera were more inhibited than those of the 3′-Me-DAB sera.4. In the findings of the starch-gel electrophoresis, the zymograms of the rachitic sera were different from those of the 3′-Me-DAB sera. The zymograms of the rachitic sera, staining by α-Naphthylphosphate, β-Glycerophosphate, p-Nitrophenylphosphate and Phenylphosphate, were fairly showed the same pattern, but the zymograms of the bone AP, staining by the same substrates, were not stained by β-Glycerophosphate.5. Significant difference was found out at the 55°C heat inactivation ratio of the AP in the sera from rachitic sera and 3′-Me-DAB sera. The ratio of the AP inactivation in the bone extracts were fairly similler to those of the rachitic sera.
- 西日本整形・災害外科学会の論文
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