Examinations on the Determination of Calcium Concentration in the Blood by Yanagizawa's Method

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Recently, I have studied on calcium determination by Yanagizawas' method. It makes use of property that Sunchromin Fast Blue M. B produces a special colour (violet) in existence of calcium in the alkaline solution.(1) Total calcium concentration: It must be taked more examinations to compare with other methods (to determine the total calcium concentration in serum), but I have believed that it is fairly reliable method for clinical determination. In 30 cases of the normal adult, the average of the total calcium concentration in serum was 10.3mg/dl.(2) diffusible calcium concentration: I could not get a satisfactory result from this method about the determination of diffusible calcium concentration. It is believed that the clinical worth is doubtful in present day.
西日本整形・災害外科学会の論文