Scanning Electron Microscopic Studies of Rabbits Spinal Cord by Resin Cracking Method
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The availability of the scanning electron microscope in revealing fine details of the Surface morphology of cells and tissues has been already established. Recently, scanning electron microscopic studies of nervous tissue have been reported on the chick spinal cord, cilia in the brain, isolated nerve fibers.However, all of these studies have treated with the surface morphology of cells and tissues, and there have been no studies performed on the inner structure, as far as is know.In the present study on the anterior horn cells, the neuroglia cells and the mylinated nerve fibers of the spinal cord as observed with the scanning electron microscope, the author attempted not only to reveal the surface morphology of cells and tissues but also their interior structure, making use of Tanaka's freezed resin cracking method.Small cubes cut from the spinal cord of the rabbit were fixed and dehydrated, and embedded in gelatin capsules filled with Cemedine 1500. The Cemedine was hardened at-30°C and the capsules were cracked in two pieces. The cracked surface of the specimen was observed under the scanning electron microscope with following results:1. In the cytoplasm of the anterior horn cell the Nissl bodies were observed like specks. The Nissl bodies were denser than the cytoplasm surrounding them and contained numerous granules (about 400 A in diameter) and membranous structures oriented parallel to each other. In the cytoplasm corresponding to the Golgi regions many vesicles and cisternal and membranous structures could be seen. On the surface of nerve cell bodies small swollen terminations of axons, which seemed to be end-bulbs, were observed.2. The membranes consisting of myelin sheaths, which surrounded the axon as a whirlpool, were observed in three dimensions. The myelin sheath sometimes emerged thinner processes which could be considered to be the cytoplasmic processes of the neurogia cell.3. The cilia covering the ependymal cells of the central canal were fairly uniform in number (12-15) from cell to cell, and were arranged in a tuft for each cell. The surface was also rich in microvilli which formed various shapes.
- 西日本整形・災害外科学会の論文
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