Establishment of Ascites Cell Tumor Induced by Schmidt-Ruppin Strain of Rous Sarcoma Virus in CDF1 Mouse:Bone Marrow Inoculation and Preservation at Low Temperature

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概要

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• Recently the solid tumor induced by Schmidt-Ruppin strain of Rous sarcoma virus was converted into the ascites form. These ascites cell tumors, approximatelly 1×105 tumor cells, were inoculated into the bone marrow of CDF1 mice through a little hole drilled below the left femur condyle. The lesion of the left femur were observed roentogenographically and histologically. After 6 or 8 weeks with the inoculation into the bone marrow, the bone destraction was seen only a little. Histologically no tumorous osteoid was found.These ascites cell tumors survive well by dipping into the tissue culture medium and by preserving in the low temperature.

• 西日本整形・災害外科学会の論文

著者

• 池本 和人

  山口労災病院

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