The Distribution of Anterior Horn Cell in Peripheral Nerve of Rat.

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To clarify the distribution of anterior horn cell in the peripheral nerves is useful to diagnose the level of spnal cord injury or cevical radiculopathy. The present report deals with the distribution in rats by the method of horseradish peroxizase (HRP) histochemistry.25 wistar rats were used in this experiment. Each periphral nerve (axillar, musculocutaneous, radial, median and ulnar nerve) was cut at the axillar area. HRP of 1250 unit was injected into the proximal stump. From 24 to 48 hours later, the rat was perfused with saline solution and fixed by 2.5% glutalaldehyde in 0.2M phosphate buffer. The C4-Th2 segment was removed and was cut in serial longitudinal sectionof 100μm thickness. The sections were stained for HRP with 3, 3′-diaminobenzidine and hydrogen peroxidase. The location and number of labeled motoneuron was studied.The labeled motoneuron of axillar and musculcutaneous nerves located at C5, 6 and the average number was 223 and 309 respectively. The motoneuron of radial, median and ulnar nerve located from C5 to Th1, C6 to Th2 and C8 to Th2, and the number was 613, 620 and 258 respectively. These results contribute the diagnose of spinal cord injury or cervcal radiculopthy.