スルメイカの外套膜筋のヘビ-メロミオシン

概要

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The method for isolation of heavy meromyosin (HMM) from squid mantle myosin was developed, and the biological properties of HMM were studied. 1) HMM was prepared by digesting squid myosin with trypsin (1/500, w/w) at 10C° for 10min, followed by addition of trypsin inhibitor. The digest was dialyzed against 0.05M KCl, pH 7.5, and centrifuged at 100, 000×g for 120min. The supernatant was used as squid HMM solution. 2) Squid HMM thus prepared was homogeneous both in ultracentrifugation and in Sephacryl S-300 gel filtration. 3) Ca-, Mg-, and EDTA-ATPases of squid HMM showed essentially the same KCl concentration, pH, and temperature dependencies as those of squed myosin. 4) Mg-ATPase of squid HMM alone was sensitive to Ca as was acto-HMM. Ca-sensitivity of HMM disappeared in the presence of 1-1.3M urea in the reaction medium.