有機酸塩による利尿の研究-醋酸-,コルク酸,アヂピン酸-,コハク酸ソーダの利尿作用と其腎内排泄部位とに就て-
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In a recent research sodium succinate has been used as an antidote against pentobarbital-intoxication, and now it is generally accepted that succinate has a remarkable diuretic action. This has promoted considerable interest for us. Including this agent, I have compared the diuretic actions of dicarboxylic acid salts in this series, that is sodium succinate, adipate and suberate while taking acetate as a standard. Japanese toads were used, throughout in this experiment for a study of diuresis. I incised skin and muscles of this animal on its back on a dry wooden board by thermocautery without anesthesia. Special cannula were inserted into the ureters on both sides, and using a Y-form tube the urine was collected into a thin glass measuring cylinder, and its amount was read every 15 minutes. With. no water-supply the urine of the animals tnus treated generally decreased slowly and rather regularly, and 2 or 3 hours later its quantity reached a level of <I>ca</I>. 0.1 cc in this time-interval. Using this level as a control, 0.5-1.0 cc of 1 normal solution or 4 cc of isotonic solutions these agents were injected into Vena cutanea magna. Soon after this injection the urine-flow increased in most cases, and usually after one hour more it returned to the control level; sometimes tp a lower level than before. This increased portion during this period was divided by injected solution's quantity and multiplied by 100, so I expressed the activity of the diuretic action of a certain pharmaca in percent-rate. As to the method for the investigation of the ratio of excreted substances through both glomeruli and tubules, I applied "modified <I>Tamura's operation</I>" on Japanese toads. The determination of the agents in toad's urine was carried out by <I>Van Slyke</I> and Palmer's method well known as the total organic acid estimation of urine, but I modified it slightly in details taking thymol blue as an indicator instead of phenolsulphophthalein and tropaelin 00. Increase of urine flow by the intravenous injection of 1 normal solution of acetate was 50.8%, succinate 82.9%, adipate 79.4% and suberate 132.6%. On the other hand, in case of isotonic solution, acetate was 25.9%, succinate 41.9%, adipate 28.2% and suberate 49.5%. The ratio of elimination of organic sodium salts from tubules as compared with that from glomeruli, by the intravenous injection of acetate was 4/100, succinate 14/100, adipate 17/100 and suberate 25/100. The diuretic actions of these salts were usually less effective When injected into Vena abdominalis which corresponds to the portal vein of warm-blooded and the liverless toads according to <I>Molitor</I> and <I>Pick's</I> method very often showed higher diuretic response to the organic salts than normal toads.
- 社団法人 日本薬理学会の論文