膜と免疫応答
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概要
- 論文の詳細を見る
The process of recognition among cells of immune system provides one of the most interesting molecular problems in membrane biology today. To solve such problems, we have investigated the immunological reactions on the membrane from various aspects. First, we have found that the binding reaction of antibodies with fluorescent lipid haptens in model membranes is dependent on the membrane fluidity, whereas the rotational motion itself is almost independent. We have also found that the binding of macrophages to lipid monolayers containing lipid haptens is dependent on the membrane fluidity, while the spreading is independent. All the results are discussed in terms of the recognition and triggering of macrophages on the membrane surfaces. Next, we have developed procedures for reconstituting transmembrane proteins into supported planar lipid monolayers. These monolayer membranes are shown to be useful for studying receptor-specific regulation of cell-mediated immune response. Last, w have studied the initial recognition steps of target tumor cells by monoclonal antibody against mouse MHC antigen. Ca<SUP>2+</SUP> influx, redistribution of membrane-bound Ca<SUP>2+</SUP> and membrane fluidity after the binding of the antibody to the target tumor cells have been well characterized by stopped-flow fluorometry.
- 日本膜学会の論文