Calcium Binding and Enzymatic Activities of Cell Membrane Isolated from Cardiac Muscle

概要

論文の詳細を見る
Cell membrane was isolated from porcine ventricles and its Ca2+ binding and ATPase activities were investigated. The cell membrane (heart sarcolemma) exhibits Na+-K+activated ATPase and Ca2+ stimulated. Mg-ATPase activities which suggest the presence of "Ca-pump"system. Ca2+ binding to the cell membrane was dependent on Ca2+ concentration and analysis of binding curve revealed that the existence of two kinds of binding sites (high and low affinity sites). Mg2+ increased Ca2+ binding at the high affinity site whereas Na+ decreased it. These actions of Mg2+ and Na+ may be physiologically important for the maintenance and transient change of intracellular Ca2+ concentration at rest and excitation of the membrane
日本膜学会の論文