:with Relation to Surface Properties and Permeability of the Membranes

概要

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The growth of mammalian cells, especially human Flow 7000 cells, on various kinds of hydrogel membranes was studied with relation to their surface properties and permeability. (1) It was demonstrated that positive charge of the membranes enhanced cell growth and that negative charge and high water content have an adverse effect. When poly (methylmethacrylate) stereocomplex membranes were employed, rather good cell growth was observed even on the membranes of high water content.Furthermore, the cell growth on the stereocomplex membranes was dependent on the ratio of isotactic polymer to syndiotactic polymer. From these results, it is reasonable to assume that the charge or other surface properties of hydrogel membranes have affected cell adhesion and growth. (2) When the fresh medium or air was perfused on the opposite side of poly (methylmethacrylate) stereocomplex membrane on which the cells were grown (perfusion method), the cells continued to grow into higher density than by the conventional static culture method. In case of medium perfusion, the cell density obtained was dependent upon both the permeability and the number of scaffolding for cell growth of membranes. (3) The Virus multiplication in the cells grown on poly (methylmethacrylate) stereocomplex membranes by perfusion method increased in proportional to the cell density
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