潅流系細胞膜での保存的成分流束の測定

概要

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Theoretical consideration was performed on the method of flux measurement of conservative ions (Na+, K+, Cl-, etc.) through the cell membrane of perfused multi-compartments system, and the experimental results of ionic fluxes of the perfused dog salivary gland were reported. The perfused system is able to divide into several conceptual compartments; i.e., the arterial, the cellular, the interstitial, the venous compartments and etc. Each compartment is able to handle as capacitor in which ions are charged or discharged by their fluxes. The capacitance (Ci) and the flux (fi) of general compartment are defined as follows, _??_ where ni is moles of ion i, q is the chemical potential, ci is concentration, and V is volume of solution in the compartment. The volume flow (iv) is shown by dVIdt. In the volume compartment, where the volume is constant, the capacitance and the flux are shown as follow. _??_In the flow compartment, where the concentration of part of solution is constant, the following equations are used. _??_ In the perfused experiment of dog salivary gland, each compartmental fluxes of ions was measured, and it was clarified that the main part of transient potassium outflux from cell at the initial phase of secretion consists of flux into the interstitial compartment.
日本膜学会の論文