STUDIES ON THE ASSAY METHOD OF MICROBIOLOGICALLY ACTIVE CONCENTRATIONS IN THE SENSITIVITY TEST MEDIA FOR ANTITUBERCULOUS AGENTS

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The thin agar diffusion method, using rapidly growing saprophytic mycobacteria or malachite-green low-sensitive bacteria as test organisms, was applied to the assay of microbiologically active concentrations in the sensitivity test media for antituberculous agents. 1) In the experiment, tested agents, basal media, applied test organisms and the resulted assayable ranges were as follows: INH, Ogawa's 3% KH2PO4 egg medium, Mycob. H-7, >0.08μg/ml. Ethambutol, Ogawa's 3% KH2PO4 egg medium, Mycob. H-7, >1μg/ml. Ethionamide, Ogawa's 3% KH2PO4 egg medium, Mycob. 238, >20μg/ml. Kirchner's agar medium, Mycob. 238, >5μg/ml. Sulfisoxazole, Ogawa's 3% KH2PO4 egg medium, Mycob. H-7 or E. coli K-12, >10mu;g/ml. Cycloserin, Kirchner's agar medium, Mycob. H-7 or E. coli K-B, >20μg/ml. Viomycin, Kirchner's agar medium, Mycob. H-7, >10μg/ml. Kanamycin, Kirchner's agar medium, Mycob. H-7, E. coli K-B or Kleb. PCI 602, >5μg/ml. Streptomycin, Ogawa's 3% KH2PO4 egg medium, Mycob. H-7 or Kleb. PCI 602, >5μg/ml. Capreomycin, Kirchner's agar medium, Mycob. 11-7, >10μg/ml. 2) Stabilities of the antituberculous agents in the sensitivity test media during storage were determined by these methods. Streptomycin and sulfisoxazole were highly stable, without any remarkable deteriorations even after 6 months' storage at 37°C. Kanamycin, ethambutol, viomycin and capreomycin kept their active concentrations at 4 but were remarkably deteriorated at 37°C during 6 months' storage. INH (5, 1μg/ml) was stable at 4°C but unstable at 37°C during 3 months. Ethiona mide, cycloserin and INH (0.1μg/ml) decreased in their active concentrations at 4°C during 3 months.
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STUDIES ON THE ASSAY METHOD OF MICROBIOLOGICALLY ACTIVE CONCENTRATIONS IN THE SENSITIVITY TEST MEDIA FOR ANTITUBERCULOUS AGENTS

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