抗酸菌の中性紅反応に関する一考察

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Modifications for the neutral red test described by Dubos and Middlebrook have been tried by many investigators, and it is recognized that the intensity of the reaction depends on the methods employed. The authors have observed that the Aratani strain of atypical group organisms gave a negative reaction for this test when the bacterial mass was pretreated with 50%methanol, while positive with absolute methanol.<BR>One handred and thirteen acid-fast bacilli, including 8 human strains, 6 bovine strains, 9 avian strains, 4 photochromogens, 21 scotochromogens, 21 nonphotochromogens, 27 rapid-growers from human sources, 17 rapid-growers from natural sources, were examined of the neutral red reaction, using 50% and 100% methanol, respectively, as a pretreatment agent.<BR>Human strains of tubercle bacilli showed much stronger reaction by the treatment with 50%methanol, whereas the majority of M. kansasii and scotochromogens showed much stronger reaction by the treatment with 100% methanol.<BR>Irrespective of the concentrations of methanol, the majority of bovine, avian and nonphotochromogenic strains showed much the same intensity of tne reaction, and the rapid-growers both from human and natural sources showed a negative reaction.
一般社団法人日本結核病学会の論文