ヘテロザート前処置マウスの結核菌感染に対する抵抗性について
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概要
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The treatment of tuberculosis with certain heterologous bacterial substances was tried for the first time about forty years ago by Susumu Nukada and his co-workers, experimentally and clinically. T h ey demonstrated that the course of experimental tuberculosis in rabbits and guinea pigs was favorably influenced by subcutaneous injections of very small doses of heated-killed typhoidgonococcal vaccine at weekly intervals. Later, the antituberculous resistance of guinea pigs and mice inoculated with a mixed autolysate of the said bacteria (Heterosate) was found to be greater than that of the animals vaccinated with the bacteria themselves. In this study, the method of administration (pretreatment) was investigated with mice, mainly with regard to the following three points: dose of the autolysate (concentration), route of inoculation (subcutaneous, intravenous, intraperitoneal), and kind of immunogen (heated vaccine, autolysate-supernatant, autolysate-sediment). Cultures of N. gonorrhoeae an d S. typhi were separately suspended in sterile distilled water, killed by heat (heated vaccine), incubated at 37°C for ten days with occasional shaking by hand. Then the suspension was centrifuged for 30 minutes at 10, 000 rpm, and the supernatant (autolysate) was used for the preparation of "Heterosate", which was made by mixing 3 volumes of gonococcal and 1 volume of S. typhi autolysate. In the present experiments were use d this original solution, 10 time diluted, and 100 time diluted solution, the sediments of each suspension as well as the heated vaccine. Mice of CF-1 and ddY strains at the 4th week of age were injected w ith each substance, three doses being given at intervals of five days. At the 10 th day after the third inoculation, a given amount of M. tuberculosis hominis strain Kurono was injected intravenously. Studies were made on the sun ival rate of the mice at the end o f the 3 rd week after infection and their survival time in comparison with those of the controls. The results were summarized as follows: 1. The enhancemen t of antituberculous resistance was most remarkable in the mice pretreated with 10 time diluted solution of the autolysate. The defensive power of the mice vaccinated with the original solution was less prominent than that of animals treated with 10time diluted solution, although the vaccinated mice showed longer survival time than the untreated controls in this experiment (Table 1).2. Concerning the route of vaccination, t h e intravenous injection of the autolysate seemed to give stronger resistance against tuberculous infection than subcutaneous or intraperitoneal treatment, but the differences among them were not significant (Table 2).3. The autolysate-supernatant increased antituberculous resistance o f mice more markedly than the heated vaccine of the bacteria. As to N. gonorrhoeae, not only its autolysate-supernatant but also the sediments showed the said resistance enhancing action (Table 3).
- 一般社団法人 日本結核病学会の論文