非定型抗酸菌に関する研究-2-生物学的性状の考察

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During the six-year period from 1966 to 1971, the author conducted 11, 431 sputum culture and obtained 382 specimens of atypical mycobacteria from 74 cases. These 74cases were subjected to the following biological property tests: 1) The pigmentation of the colonies on the Ogawa eg g medium proved to be orange, orange yellow, light orange, light yellow white, greyish white and white. The most frequent was greyish white followed by orange, while the least frequent were orange yellow and white. The difference in the color tone was surveyed with an automatic spectrophotometer (HITACHI ESP 3T), but no conspicuous results were obtained since all the colors showed a similar curve with 450 mμ at its peak. When petro-ether and acetone were employed to abstract the pigments, those of photochromogens could easily be abstracted while it took considerably longer time in the other groups. 2) Most of the colonies on the Ogawa egg medium were roun d in shape and only a few presented other forms. However, because of the great number of bacteria on the egg medium, there were many cases whose form could not be identified. 3) As for the smoothness of the colonies, most of the atypical mycobacteria showed smooth colonies, whereas a part of the photochromogens and nonphotochromogens showed a rough form. 4) When the b acteria were observed under a microscope by Ziehl-Neelsen staining, the photochromogens proved to be long bacilli, while the nonphotochromogens and all the others were short bacilli. 5) Some of the photochromogens presented inconspicuous cord formation while the others did not. 6) As fo r the temperature of culture, all atypical mycobacteria showed growth at 37°C, and only one case of nonphotochromogens did not grow at 25°C. Comparing the grade of colour production after exposure to light at different. Culture temperature, the grade was more marked at 37°C.
一般社団法人日本結核病学会の論文

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正井秀雄
関東中央病院臨床検査科

非定型抗酸菌に関する研究-2-生物学的性状の考察

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