SERUM ANTIMYCOBACTERIAL ACTIVITY TEST FOR CHEMOTHERAPY OF THE PATIENTS INFECTED WITH UNCLASSIFIED MYCOBACTERIA

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概要

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• The unclassified mycobacteria, especially, scotochromogens and nonphotochromogens are more resistant than M. tuberculosis to streptomycin, isoniazid and para-aminosalycylic acid. Today the major problem in the chemotherapy is the formulation of drug regimens for the treatment of the patients infected with unclassified mycobacteria which have become resistant to the primary anti-tuberculous drugs.<BR>The "specific serum antimycobacterial activity test" described by Dye and Kass has been considered as useful for the formulation of the drug treatment of tuberculous patients.<BR>This paper presents the study on modified method of 2-hours serum antimycobacterial activity test to obtain adequate chemotherapy regimens for unclassified mycobacterial patients. Materials and methods<BR>1. Unclassified mycobacteria were cultured from sputum or resected lung specimens. The strains used were as follows:<BR>scotochromogens: Nagashima, Ariga, Mitsui A, Uemura, Aoki and Ishii<BR>nonphotochromogens: Mitsui B, Hikichi, Tanaka, Kamamori and Shirakashi<BR>2. Serum:<BR>serum of normal adults<BR>serum of tuberculous patients under chemotherapy<BR>serum of patients infected with unclassified mycobacteria<BR>3. The bioassay method is as follows: The serum is diluted with Kirchner liquid medium, to make a series of tubes with dilutions of 1: 4, 1: 8, 1: 16, 1: 32 and 1: 64.<BR>Each tube is inoculated with 0.01 mg of unclassified mycobacteria. These tubes are incubated at 37°C for three days. At the end of this period, one drop of 0.05% Resazurin solution is added to each tube. The tubes with dye are incubated at 37°C for twenty-four hours. The color of the control tube with mycobacteria becomes pink by the reduction of Resazurin. In the tube containing serum with inhibitory activity, the color remains either blue or purple by the complete or incomplete prevention of the reduction of Resazurin. The highest dilution of serum which prevented the reduction of Resazurin to purple is regarded as the titer of antimycobacterial activity.<BR>Results<BR>Sera of normal adults did not inhibit the growth of unclassified mycobacteria.<BR>Sera obtained from the patients who received SM, INH and PAS scarcely inhibited the growth of all scotochromogens and nonphotochromogens.<BR>The serum activity, to unclassified mycobacteria, 2 hours after the drug administration was higher than that after 4 hours.<BR>The chemotherapy with KM plus EB plus INH or TH will be successful in the treatment of the disease caused by unclassified mycobacteria.<BR>It is believed that this test can serve as a guide to the effective antimycobacterial treatment for the patients infected with unclassified mycobacteria.

• 一般社団法人 日本結核病学会の論文

著者

• 中村 善紀

  日本鋼管清瀬浴風院

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