NTIMYCOBACTERIAL ACTIVITY OF SERUM FROM PATIENT UNDER CHEMOTHERAPY

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2 ml of bacterial suspension of tubercle bacilli in Dubos liquid medium were mixed with thesame amount of melted agar in a Widat test tube. Superposition of drug solution upon the agar plug inhibited the bacilli to grow in the uppermost layer of the agar culture. The bacilli grew in the depth and formed a colonial disc below the inhibitory zone. The same was observed with superposition of serum from patient under chemotherapy. Thedepth of the inhibitory zone can indicate the antimycobacterial activity of drug or serum. These observations were reported by us previously in detail. The purpose of this paper is to report the subsequent results obtained from our antimyco- bacterial activity test of patients' sera under usual chemotherapy. The maximum dilution ofserum for minimum inhibition was measured. The serum was tested 6hrs after administrationof drugs. The results are as follows. In the following description "SM 1.0g+INH 0.1 g" or the like will mean a serum froma patient who was given SM 1.0 g+INH 0.1 g or the like. 8× (+) will mean that the serumis active up to 8 fold dilution. 2× (-): the serum is inactive with its 2 fold dilution. 1. Most of "SM 1.0 g+INH 0.1 g" or "PAS 10/3 g+INHG 0.3g" showed 8× (+). None ofthem was 2× (-)."INH 0.1 g +PAS 10/3 g" or "INH 0.1 g+CS 0.25 g" were mostly 2× (+) × (+). Some ofthem were 2× (-)."PAS 10/3 g+INH 0.2 g" is more active than "PAS 10/3 0.1 g". Most of "PAS 10/3 g +TH 0.3 g" were more active than "PAS 10/3 g+INH 0.2 g"."CS 0.25 g alone" were 2× (-) with few exceptions. Nevertheless it is worthy of note that"CS 0.25 g;+INH 0.1 g" is comparable to "PAS 10/3 g+INH 0.1g"."PZA 1.0 g alone" were also 2× (-)."PZA 1.0 g+INH 0.2 g" or PZA 1.0 g+INHG 0.5 gshowed activity. 2. In the same patient, "PAS+PINHG" was stronger than. "PAS+TH". But, in a few cases, it was reverse. In every case, "PAS+INHG" was stronger than "PZA+INHG". In a few cases, every combination examined gave a strong activity.On the contrary, also in a few cases, every combination gave only a weak activity.3. Antimycobacterial activity of CS and TH.With 1.5 γ/ml TH superposition, in a week, the agar plug brought up bacterial colonies in the top layer of it. With 3.1 γ/ml TH superposition, it brought up colonial disc in a deeplayer, about 0.7 cm from the surface. With 6. 2 γ/ml, 12.5γ/ml the distance was 1.3 cm and1.5 cm respectively. In 10-14 days, another colonial disc appeared in the previous inhibitory zone. The same was observed in a case of CS 50 γ/ml or "PASH+TH" superposition. These phenomena suggested the differences between TH, CS and SM, KM, VM, PAS, INH. 4. The serum antimycobacterial activity demonstrated in this way was considered to beattributed to the drugs administered. The test was considered to be usefull in evaluatingantimycobacterial activity of serum from patient under chemotherapy.
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NTIMYCOBACTERIAL ACTIVITY OF SERUM FROM PATIENT UNDER CHEMOTHERAPY

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