結核菌培養早期証明法の考察-3-

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As for the materials to be examined, 18 samples of pus, 19 of urine and 21 of pleural fluid, of which the smears were all negative of tubercle bacilli, were treated with 4% solution of sodium hydroxyde and an equal or doubled amount of Kirchner's medium was mixed to the washed sediment. A drop of the washed sediment suspended in Kirchner's medium was placed on a slide. The slide was kept in the moist chamber and incubated. The slide was taken out from the moist chamber after 2 to 3 days, then it was dried and stained. A tube coutaining the same suspension of the washed sediment in Kirchner's medium was sealed and incubated. The tube was centrifuged and the sediment was smeared on a slide for staining.
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