PREPARATION AND APPLICATION OF P32-LABELED TUBERCLE BACILLI
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概要
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P<SUP>32</SUP>-labeled tubercle bacilli (Aoyama B strain) were obtained from the cultures grown on the Kirchner's protein-free medium containing Na<SUB>2</SUB>HP<SUP>32</SUP>O<SUB>4</SUB>. The labeled bacilli were injected into <I>vena auricularis</I> or <I>vela mesenterica</I> of norni: 1 rabbits and rabbits previously inoculated with <I>M. tuberculosis var. hominis</I> for the observation of the distribution of P" in the tissues of various organs 30 min., 24 hrs., and 96 hrs. respectively after injection. The larger amounts of the labeled bacilli were demonstrated in the lung when the injection was performed into <I>vena auricularis</I>, and in the liver when injected into <I>vena mesenterica, </I> and there was no difference between normal and tuberculous rabbits. On the other hand, fine crystals of ammonium phospho-niolybdate containing P<SUP>32</SUP> which had been injected in the same manner, were demonstrated in the liver, lung and kidney in an almost equal amount regardless to the route of injection, and also there was no difference between normal and tuberculous rabbits. Furthermore, the P<SUP>32</SUP>-labeled tubercle bacilli were distributed mainly in the lung of both normal rabbits and rabbits with strongly sensitized lungs and the same results were obtained in the case using a small amount of the labeled bacilli as well as the large amount. Consequently, we assumed that the distribution of the tubercle bacilli and ammonium phospho-molybdate in the organs of animals depends upon the physical and mechanical conditions such as the size or surface activity of the injected material. The "localization of antigen by the antigen-antibody reaction" which has been believed since Roessle, was not remarkable in the tuberculous allergy reaction.<BR>Furthermore, in order to know the ratio of nonlabeled phosphate to P<SUP>32</SUP>-phosphate added in the medium for the cultivation of the best radio-active tubercle bacilli, the tubercle bacilli (avian type) were cultured for 7 days on kirchner's protein-free media which contained varying amounts of non-labeled phosphate and 50μc P<SUP>32</SUP>-phosphate. The radio-activity of the cells collected was compared. Consequently, the most radioactive P<SUP>32</SUP>-labeled bacilli were obtained from the medium in which 1/16 of the usual amount of non-labeled phosphate was used. The bacterial yield from this medium was the same as usual.
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